er her2 breast cell line t47d  (ATCC)

Journal: bioRxiv

Article Title: Direct Synthesis of Targeted Nanosized ICG J-aggregate for Photoacoustic Imaging

doi: 10.64898/2026.05.12.724349

Figure Lengend Snippet: (a) Schematic of copper-free click chemistry wherein compounds such as DBCO-PEG-folate (D-P-FA), DBCO-biotin (D-biot), streptavidin (Strep), and biotin_PEG-folic acid (Biot-P-FA) are conjugated to the surface of the nJAAZ particles onto the available azide groups ( in orange ) via direct conjugation or via streptavidin-biotin conjugation. (b) Zeta potential magnitude of different molar ratios of bare and DBCO-PEG-folate functionalized nJAAZ particles for different ICG-N 3 :ICG molar ratios. (c) Size of the different molar ratio JAAZ particles before and after functionalization with DBCO-PEG-folate. (n=3 for b and c (above), ns=non-significant, *= p<0.05, **= p<0.01, ***= p<0.001, **** p< 0.0001). (d). Representative SPR binding curves for the binding of nJAAZ-FA at 0.4, 1, and 10 µM on FOLR grafted on the SPR sensor. (e). Confocal fluorescence imaging of T47D cells incubated with various nJAAZ, fluorescently labelled nJAAZ, and fluorescently labelled nJAAZ-FA.

Article Snippet: Estrogen Receptor-positive/HER2-negative ER+/HER2-breast cell line T47D was obtained from the American Type Culture Collection (ATCC).

Techniques: Conjugation Assay, Zeta Potential Analyzer, Binding Assay, Fluorescence, Imaging, Incubation

Journal: Clinical Cancer Research

Article Title: Tamoxifen Response at Single-Cell Resolution in Estrogen Receptor–Positive Primary Human Breast Tumors

doi: 10.1158/1078-0432.CCR-23-1248

Figure Lengend Snippet: T47D response to tamoxifen at a single-cell level. A, Illustration showing T47D scRNA-seq workflow. B, UMAP plot of T47D scRNA-seq cells color coded by groupA (luminal A–like subpopulation) or groupB (luminal B–like subpopulation). C, UMAP plot of T47D scRNA-seq cells color coded by treatment condition. D, Volcano plot showing enriched and depleted gene sets after tamoxifen treatment in T47D cells. Significant gene sets were defined as a gene ratio of >0.125 and a log 10 q value of <−4.5 or >4.5, and a thresholding limit of 10 was applied when log 10 q value >10 for visualization. E, Feature plots showing the expression of luminal epithelial markers and proliferation score in T47D cells.

Article Snippet: We obtained the ER + /HER2 − cell line T47D from the ATCC.

Techniques: Expressing

Journal: Clinical Cancer Research

Article Title: Tamoxifen Response at Single-Cell Resolution in Estrogen Receptor–Positive Primary Human Breast Tumors

doi: 10.1158/1078-0432.CCR-23-1248

Figure Lengend Snippet: Targeted analysis of four tamoxifen-responsive tumor pairs. A, UMAP plot of scRNA-seq cells from four tumors that demonstrated depletion of scTAM-response-T47D signature. Cells are color coded by cell type. B, UMAP plot of scRNA-seq cells from four tumor pairs, color coded by tumor and treatment condition. C, Heat map of upregulated and downregulated gene sets in tamoxifen-treated tumor cells relative to control cells. D, Volcano plot showing enriched and depleted gene sets after tamoxifen treatment in malignant cells from 4 ER + /HER2 − tumor pairs. Significant gene sets were defined as a gene ratio of > 0.085 and a log 10 q value of < −10 or > 10 for visualization. E, Kaplan–Meier (KM) curve for overall survival using two independent clinically annotated datasets with transcriptional data. ER + /HER2 − patients treated with endocrine therapy were assigned a centroid score of our malignant cell-specific tamoxifen resistance signature (scTAM-resistance-M) and stratified by high and low score. High signature score is associated with significantly worse overall survival in patients in METABRIC (HR, 1.63; P = 0.023) and SCAN-B (HR, 2.94; P = 0.002). Statistical significance was assessed by the log-rank test and the estimates of survival probabilities and cumulative hazard with a univariate Cox proportional hazards model.

Article Snippet: We obtained the ER + /HER2 − cell line T47D from the ATCC.

Techniques: Control

Journal: Clinical Cancer Research

Article Title: Tamoxifen Response at Single-Cell Resolution in Estrogen Receptor–Positive Primary Human Breast Tumors

doi: 10.1158/1078-0432.CCR-23-1248

Figure Lengend Snippet: Identification and characterization of resistant tumor cell subpopulations in four tamoxifen-responsive tumor pairs. A, Individual cells were assigned a score from the T47D tamoxifen response signature compared with cluster matched untreated score. Application of response score to UMAP plot demonstrated three distinct clusters with enriched signature score on treatment (cluster 3, 12, 19) and one tamoxifen-sensitive cluster with depletion of response score (cluster 2). B, Abundance of cells from resistant clusters in each of the four tumors. C, Stacked bar chart showing distribution of cells within 22 distinct clusters, color coded by tumor and treatment condition. D, Kaplan–Meier (KM) curve of the cluster 19 signature (scTAM-resistance-C19), using ER + /HER2 − patients that received endocrine therapy from METABRIC. Higher signature score predicted worse overall survival (HR, 2.17; P < 0.001). Survival curve differences were calculated by the log-rank test and the estimates of survival probabilities and cumulative hazard with a univariate Cox proportional hazards model. E, We obtained data of clinically annotated endocrine therapy sensitive and resistant tumors from Xia and colleagues . All three tamoxifen resistance signatures were enriched in resistant tumors compared with sensitive. Significance was determined using the Wilcoxon rank-sum test, ***, P < 0.001.

Article Snippet: We obtained the ER + /HER2 − cell line T47D from the ATCC.

Techniques:

Journal: Cellular Oncology (Dordrecht)

Article Title: AKT-driven epithelial-mesenchymal transition is affected by copper bioavailability in HER2 negative breast cancer cells via a LOXL2-independent mechanism

doi: 10.1007/s13402-022-00738-w

Figure Lengend Snippet: Basal levels of cuproproteins in different breast cancer cell lines and their modulation following TRIEN treatment. Cell extracts obtained from MDA-MB-231, SUM159, T47D and SK-BR-3 were applied to SDS–PAGE followed by Western blot with monoclonal antibodies for the detection of basal levels of (a) ATP7A, LOXL2, CCS and of the subunit II of cytochrome c oxidase. The level of CCS and the of the subunit II of cytochrome c oxidase, was measured by Western blot ( left panels; correspondent densitometric analysis in the right panels ), following 24 or 48 h of 125 µM TRIEN treatment in (b) MDA-MB-231, (c) SUM159, (d) T47D, and (e) SK-BR-3 cells. Twenty micrograms of proteins were loaded on each lane. Vinculin was used as loading control. One representative blot is shown for each antigen out of at least three independent experiments. (f, upper panel) . SOD1 activity was assessed in the different cell lines lysates using an in-gel assay, under non-denaturing, SDS-free conditions, after 48 h of treatment with 125 µM TRIEN; fifty micrograms of proteins were applied to each lane. One representative SOD1 activity assay is shown out of at least three different experiments; (f, lower panel) densitometric analysis of the activity assay. Data are presented as a mean ± SEM. Student's t-test *p < 0.05, **p < 0.01 with respect to the untreated cells only

Article Snippet: The human triple negative breast cancer (TNBC) cell line MDA-MB-231, the HER2-overexpressing SK-BR-3 cell line and the HER2 − PR + ER + T47D cell line, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA, cat. no. HTB-26TM, HTB-30TMand HTB-133TM, respectively).

Techniques: SDS Page, Western Blot, Bioprocessing, Control, Activity Assay

Journal: Cellular Oncology (Dordrecht)

Article Title: AKT-driven epithelial-mesenchymal transition is affected by copper bioavailability in HER2 negative breast cancer cells via a LOXL2-independent mechanism

doi: 10.1007/s13402-022-00738-w

Figure Lengend Snippet: Cu bioavailability modulates the level of the epithelial to mesenchymal transition hallmarks. The basal levels of fibronectin, E-cadherin, αSMA and vimentin were measured in cell extracts from ( a ) MDA-MB-231, SUM159, T47D and following 24 h exposure to 125 µM TRIEN for ( b ) MDA-MB-231, ( c ) SUM159, ( d ) T47D and 48 h after TRIEN treatment for ( e ) SK-BR-3. Twenty micrograms of proteins were loaded on each lane. Vinculin or actin were used as loading controls. ( f ) Immunofluorescence detection of E-cadherin in MDA-MB-231, SUM159, T47D 24 h after TRIEN exposure and SK-BR-3 cells 48 h after treatment with 125 µM TRIEN (upper panels) and its corresponding signal analysis (lower panels). ( g ) Fibronectin immunofluorescence of T47D and SUM159 (left panels) and its corresponding signal analysis (right panels) . ( h ) αSMA immunofluorescence in MDA-MB-231 and SK-BR-3 (left panels) and its corresponding signal analysis (right panels) . Phalloidin was used to label F-actin whilst nuclei were labeled with DAPI. Calibration bars corresponds to 100 μm in the immunofluorescence images; 40 × magnification. One representative blot/image and the corresponding signal analysis is shown for each antigen, out of at least three independent experiments. Student's t-test * p < 0.05, ** p < 0.01 and *** p < 0.001 with respect to the untreated cells only. Data are presented as a mean ± SEM

Article Snippet: The human triple negative breast cancer (TNBC) cell line MDA-MB-231, the HER2-overexpressing SK-BR-3 cell line and the HER2 − PR + ER + T47D cell line, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA, cat. no. HTB-26TM, HTB-30TMand HTB-133TM, respectively).

Techniques: Immunofluorescence, Labeling

Journal: Cellular Oncology (Dordrecht)

Article Title: AKT-driven epithelial-mesenchymal transition is affected by copper bioavailability in HER2 negative breast cancer cells via a LOXL2-independent mechanism

doi: 10.1007/s13402-022-00738-w

Figure Lengend Snippet: Cu chelation modulates the activation of the AKT/GSK3β/SNAIL axis. (a, left panel ) Western Blot of phosphorylated AKT at Ser473 (pAKT Ser473), total AKT (AKT), phosphorylated ERK1/2 (pERK1/2) and total ERK 1/2 (ERK1/2) in MDA-MB-231, SUM159, T47D and SK-BR-3 following 24 h exposure to 125 µM TRIEN for MDA-MB-231, SUM159, T47D and 48 h after TRIEN treatment for SK-BR-3. (a, right panel) densitometric analysis of phosphorylated AKT at Ser473 residue (pAKTSer473)/total AKT. (b, left panel) Western blot and (b, right panel) densitometric analysis of pAKT at Thr308 (pAKT Thr308) and total AKT (AKT) in MDA-MB-231; (c, left panel) Western blot and (c, right panel) densitometric analysis of phosphorylated GSK3β at Ser9 (pGSK3β Ser9) and SNAI1 in MDA-MB-231and (d) SK-BR-3 cells. Twenty micrograms of proteins were loaded on each lane. Vinculin was used as loading control. One representative blot is shown for each antigen, data are presented as a mean ± SEM ( n ≥ 3, Student's t-test * p < 0.05; ** p < 0.01 *** p < 0.001). (e) The level of the EMT-TFs SNAI1, TWIST1, SNAI2 and ZEB1 transcripts in MDA-MB-231 cells were evaluated by qPCR analysis following 24 h exposure to 125 µM TRIEN. Results were expressed with respect to the control (vehicle), defined as 1. Data were reported as mean ± SEM of three independent measurements (* p < 0.05, ** p < 0.01 vs vehicle)

Article Snippet: The human triple negative breast cancer (TNBC) cell line MDA-MB-231, the HER2-overexpressing SK-BR-3 cell line and the HER2 − PR + ER + T47D cell line, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA, cat. no. HTB-26TM, HTB-30TMand HTB-133TM, respectively).

Techniques: Activation Assay, Western Blot, Residue, Control

Journal: Cellular Oncology (Dordrecht)

Article Title: AKT-driven epithelial-mesenchymal transition is affected by copper bioavailability in HER2 negative breast cancer cells via a LOXL2-independent mechanism

doi: 10.1007/s13402-022-00738-w

Figure Lengend Snippet: Graphical representation of the hypothetical mechanisms proposed for the regulation by copper of proteins/kinases involved in Her2 negative and Her2-overexpressing breast cancer cells aggressiveness

Article Snippet: The human triple negative breast cancer (TNBC) cell line MDA-MB-231, the HER2-overexpressing SK-BR-3 cell line and the HER2 − PR + ER + T47D cell line, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA, cat. no. HTB-26TM, HTB-30TMand HTB-133TM, respectively).

Techniques: